1. Effects of carotenoids from Deinococcus radiodurans on protein oxidation.
- Author
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Tian, B., Sun, Z., Shen, S., Wang, H., Jiao, J., Wang, L., Hu, Y., and Hua, Y.
- Subjects
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CAROTENOIDS , *DEINOCOCCUS radiodurans , *PROTEINS , *OXIDATION , *BLOOD proteins - Abstract
Aims: To evaluate the antioxidant effect of carotenoids from Deinococcus radiodurans on protein. Methods and Results: Deinococcus radiodurans strain R1 (ATCC 13939) and its mutant strain R1ΔcrtB were used for this study. The total carotenoids (R1ex) from D. radiodurans were obtained by extraction with acetone/methanol (7 : 2, by vol), and their antioxidant activity was measured using the DPPH˙ (2,2-diphenyl-1-picrylhydrazyl) system. The protein oxidation level, in vitro and in the cell, was measured using the DNPH (2,4-dinitrophenyl hydrazine) method. The carotenoid extract R1ex scavenged 40·2% DPPH˙ radicals compared to β-carotene (31·7%) at a concentration of 0·5 mg ml−1. The intracellular level of protein oxidation in mutant R1ΔcrtB, which does not contain carotenoid, was 0·0212 mmol mg−1 protein which is significantly greater than that in the wild type (0·0169 mmol mg−1 protein) following the treatment with H2O2. The purified major carotenoid product (deinoxanthin) from the wild type showed a greater inhibition of oxidative damage in bovine serum albumin than lycopene or lutein. Conclusions: Carotenoids prevent protein oxidation and contribute to the resistance to cell damage in D. radiodurans. Significance and Impact of the Study: Our results provide the evidence that carotenoids can protect proteins in D. radiodurans against oxidative stress. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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