1. Growth of xenotransplanted leukemia cells is influenced by diet nutrients and is attenuated with 2-deoxyglucose
- Author
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Masakazu Nitta, Ichiro Hanamura, Motonori Mizutani, Mayuko Gotou, Hidetsugu Mihara, Kazuto Suganuma, Norikazu Tsunekawa-Imai, Mineaki Goto, Hiroshi Miwa, Akira Imamura, Masaya Watarai, Hidesuke Yamamoto, Motohiro Wakabayashi, Miyuki Takahashi, Hiroko Komatsubara, Shohei Mizuno, Tomohiro Horio, and Masato Shikami
- Subjects
Cancer Research ,medicine.medical_specialty ,Programmed cell death ,Antimetabolites ,Blotting, Western ,Transplantation, Heterologous ,Mice, SCID ,Biology ,Deoxyglucose ,Immunoenzyme Techniques ,chemistry.chemical_compound ,Mice ,Mice, Inbred NOD ,Internal medicine ,medicine ,Tumor Cells, Cultured ,Animals ,Humans ,Glycolysis ,Beta oxidation ,Cell Proliferation ,chemistry.chemical_classification ,Leukemia, Experimental ,Fatty acid ,Hematology ,medicine.disease ,Diet ,Leukemia ,Endocrinology ,Enzyme ,Oncology ,chemistry ,Dietary Supplements ,Female ,Energy Metabolism ,Etomoxir ,Neoplasm Transplantation - Abstract
We examined the effects of diet nutrients on xenotransplanted leukemia cells, THP-1 or NB4. THP-1 tumors showed more growth when fed with high fat diet, while NB4 tumors grew more with high carbohydrate diet. Then, administration of 2-deoxyglucose (a glycolysis inhibitor) showed a significant antitumor effect on both tumors: NB4 tumor showed large necrotic areas, while THP-1 tumor did not, but had augmented expression of enzymes for fatty acid oxidation. 2-Deoxyglucose inhibited the growth of NB4 by cell death because main energy producing pathway (glycolysis) was abolished, while 2-deoxyglucose slowed the growth of THP-1 by shifting energy metabolism to fatty acid β-oxidation.
- Published
- 2013