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The antioxidant capacity of cell-free extracts from Lactobacillus plantarum NJAU-01 obtained by using different cell disruption methods was determined. The efficacy of various methods, including high-pressure treatment (HIP), lysozyme combined with ultrasound treatment (LCU), glycine treatment (GLY), and frozen-thawed treatment (FAT) was studied. The results showed that the bacterial suspension in the HIP group visibly had the least intact cells per visual field compared with the other groups, followed by the LCU group. The HIP group possessed 5.12 lg CFU/mL viable bacteria, which was significantly lower than that of the other groups after cell disruption from the initial 9 lg CFU/mL. The protein concentration of the cell-free extract from the HIP group was higher than that of the other groups with abundant protein bands and band intensities. The HIP group presented significantly higher total antioxidant capacity and superoxide dismutase and glutathione peroxidase activities than the other groups. Our data suggest that HIP can be a preferable method to obtain cell-free extracts with excellent antioxidant capacity for further exploring the underlying antioxidant mechanism of Lactobacillus plantarum NJAU-01. ● Four methods are used for cell wall disruption of Lactobacillus plantarum NJAU-01. ● High pressure group had visibly less intact cell compared to other groups. ● The high pressure treatment released more proteins form L. NJAU-01. ● The cell-free extract by high-pressure treatment exhibited most antioxidant capacity. [ABSTRACT FROM AUTHOR]

The present study evaluated whether acute consumption of freeze concentrated yerba mate ( Ilex paraguariensis A. St. Hil.) infusion (CM) promotes improvement on the antioxidant and lipid peroxidation parameters in healthy individuals when compared to the traditional mate infusion (TM). In this cross-over study, 31 fasted volunteers ingested 200 mL of TM, CM, or water (control). Blood samples were withdrawn before and 1 h after beverages intake for the measurement of the antioxidant enzyme activities, catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx), reduced glutathione (GSH), lipid peroxidation (LOOH), and antioxidant capacity of serum (FRAP assay). CM intake promoted significant enhancement of the CAT (28.7%), SOD (21.3%), and GPx (9.6%) activities, and increased the levels of GSH (8.8%) and serum antioxidant capacity (7.5%), while TM increased only GSH values (8.3%). Serum levels of LOOH did not change significantly after consumption of both mate infusions. Acute consumption of CM by healthy individuals improved the blood antioxidant defense systems more efficiently than the traditional yerba mate and did not promote pro-oxidant effects. [ABSTRACT FROM AUTHOR]