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Dendrobium has great medicinal value, and their active components vary across different species of Dendrobium. In this study, a two-dimensional ion mobility mass spectrometry point image based on retention time and drift time was first proposed, and the collision cross-section values of Dendrobium metabolites were calculated by the IM browser, which can identify the targeted compounds more accurately, especially for isomeric compounds. During the experimental process, a total of 74 batches of Dendrobium samples containing nine species of Dendrobium were analyzed by ultrahigh-performance liquid chromatography coupled with ion mobility quadrupole-time-of-flight mass spectrometry. Nontargeted plant metabonomics combined with multivariate statistical analysis was used to realize the clustering of different species of Dendrobium , which made the identification more scientific. Therefore, this method can realize the analysis of multiple active components in Dendrobium , can be used for the identification of Dendrobium teas and can provide a reference for the quality control of Dendrobium. • Two-dimensional IM-MS point image technology was first proposed. • UHPLC-IM-Q-TOF MS was first used for the determination of plant teas. • Nontargeted metabonomics was used for the identification of Dendrobium species. [ABSTRACT FROM AUTHOR]