Search

Background Rapid elimination of Plasmodium falciparum malaria in Cambodia is a goal with both national and international significance. Transmission of malaria in Cambodia is limited to forest environments, and the main population at risk consists of forest-goers who rely on forest products for income or sustenance. The ideal interventions to eliminate malaria from this population are unknown. Methods In two forested regions of Cambodia, forest-goers were trained to become forest malaria workers (FMWs). In one region, FMWs performed mass screening and treatment, focal screening and treatment, and passive case detection inside the forest. In the other region, FMWs played an observational role for the first year, to inform the choice of intervention for the second year. In both forests, FMWs collected blood samples and questionnaire data from all forest-goers they encountered. Mosquito collections were performed in each forest. Results Malaria prevalence by PCR was high in the forest, with 2.3–5.0% positive for P. falciparum and 14.6–25.0% positive for Plasmodium vivax among forest-goers in each study site. In vectors, malaria prevalence ranged from 2.1% to 9.6%, but no P. falciparum was observed. Results showed poor performance of mass screening and treatment, with sensitivity of rapid diagnostic tests equal to 9.1% (95% CI 1.1%, 29.2%) for P. falciparum and 4.4% (95% CI 1.6%, 9.2%) for P. vivax. Malaria infections were observed in all demographics and throughout the studied forests, with no clear risk factors emerging. Conclusions Malaria prevalence remains high among Cambodian forest-goers, but performance of rapid diagnostic tests is poor. More adapted strategies to this population, such as intermittent preventive treatment of forest goers, should be considered.

International audience; Background: Madagascar is a malaria-endemic country with an increase in cases in recent years. In vector control using insecticide, a susceptible strain is necessary to evaluate insecticide efficacy, either for spraying or on nets. The susceptibility of Anopheles arabiensis from Antananarivo, Madagascar to two organophosphate, three pyrethroid, two carbamate, and one organochlorine insecticides was investigated. Since 2010, An. arabiensis strain has been maintained away from insecticide source during 110 generations with optimal insectarium conditions. Methods: Bioassay were performed on adult mosquitoes to assess the susceptibility of An. arabiensis to insecticideimpregnated papers (malathion 5 %, fenitrothion 1 %, deltamethrin 0.05 %, permethrin 0.75 %, alphacypermethrin 0.05 %, bendiocarb 0.1 %, propoxur 0.01 %, and DDT 4 %) following World Health Organization Pesticide Evaluation Scheme guidelines. Bioassay using Center for Disease Control bottle tests were also used to detect mortality. Molecular assay were carried out to detect the presence of knock down resistance (kdr) mutation using PCR techniques. Results: Anopheles arabiensis is fully susceptible with 100 % mortality to malathion, fenitrothion, deltamethrin, permethrin, alphacypermethrin, bendiocarb, propoxur, and DDT. No kdr gene was detected using PCR method. Conclusion: The strain An. arabiensis maintained in the insectarium of Institut Pasteur de Madagascar is a fully susceptible strain and can be used for insecticide evaluation.