1. FGL-functionalized self-assembling nanofiber hydrogel as a scaffold for spinal cord-derived neural stem cells.
- Author
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Wang, Jian, Zheng, Jin, Zheng, Qixin, Wu, Yongchao, Wu, Bin, Huang, Shuai, Fang, Weizhi, and Guo, Xiaodong
- Subjects
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MOLECULAR self-assembly , *NANOFIBERS , *HYDROGELS , *NEURAL stem cells , *SPINAL cord , *TISSUE scaffolds - Abstract
A class of designed self-assembling peptide nanofiber scaffolds has been shown to be a good biomimetic material in tissue engineering. Here, we specifically made a new peptide hydrogel scaffold FGLmx by mixing the pure RADA 16 and designer functional peptide RADA 16 -FGL solution, and we analyzed the physiochemical properties of each peptide with atomic force microscopy (AFM) and circular dichroism (CD). In addition, we examined the biocompatibility and bioactivity of FGLmx as well as RADA 16 scaffold on spinal cord-derived neural stem cells (SC-NSCs) isolated from neonatal rats. Our results showed that RADA 16 -FGL displayed a weaker β-sheet structure and FGLmx could self-assemble into nanofibrous morphology. Moreover, we found that FGLmx was not only noncytotoxic to SC-NSCs but also promoted SC-NSC proliferation and migration into the three-dimensional (3-D) scaffold, meanwhile, the adhesion and lineage differentiation of SC-NSCs on FGLmx were similar to that on RADA 16 . Our results indicated that the FGL-functionalized peptide scaffold might be very beneficial for tissue engineering and suggested its further application for spinal cord injury (SCI) repair. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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