1. Electrochemical immunosensor based on ZnO nanorods-Au nanoparticles nanohybrids for ovarian cancer antigen CA-125 detection
- Author
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Paulo Inácio da Costa, João Paulo de Campos da Costa, Talita Mazon, Gisane Gasparotto, and Maria Aparecida Zaghete
- Subjects
Working electrode ,Materials science ,Nanoparticle ,Metal Nanoparticles ,Bioengineering ,Nanotechnology ,02 engineering and technology ,Biosensing Techniques ,01 natural sciences ,Biomaterials ,medicine ,Hydrothermal synthesis ,Humans ,Immunoassay ,Ovarian Neoplasms ,Nanotubes ,medicine.diagnostic_test ,010401 analytical chemistry ,Substrate (chemistry) ,Reproducibility of Results ,Electrochemical Techniques ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Mechanics of Materials ,Colloidal gold ,CA-125 Antigen ,Electrode ,Nanorod ,Female ,Gold ,0210 nano-technology - Abstract
In this research, ZnO nanorods - Au nanoparticles nanohybrids have been fabricated and employed to sensitive electrochemical strategy for the specific detection of the ovarian cancer antigen CA-125/MUC126. The microdevice was developed in our lab based on gold and silver electrodes sputtered on glass substrate. The ZnO nanorods arrays were grown on working electrode using assisted microwave hydrothermal synthesis than gold nanoparticles (Au NPs) were deposited by sputtering. The Au NPs onto ZnO nanorods surface provides a favorable platform for efficient loading of anti-CA-125 antibody via binding with cystamine and glutaraldehyde. The effective loading of the biological material (CA-125 antibody and antigen) on the matrix was observed by SEM images. The electrochemical immunosensor shows a sensitive response to ovarian cancer antigen recombinant human CA-125/MUC126 with detection of 2.5ng/μL, 100 times lower than immunoblot system. Due to high specificity, reproducibility and noteworthy stability, the developed sensor will provide a sensitive, selective and convenient approach to be used to detect CA-125/MUC126.
- Published
- 2016