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Start Over Author "Dolle, Pascal" Journal mechanisms of development
18 results on '"Dolle, Pascal"'

12. A new mouse member of the Wntgene family, mWnt-8, is expressed during early embryogenesis and is ectopically induced by retinoic acid

Author

Bouillet, Philippe, Oulad-Abdelghani, Mustapha, Ward, Simon J., Bronner, Sylviane, Chambon, Pierre, and Dolle´, Pascal

Abstract

We have identified a novel mouse Wntgene using a cDNA differential screening procedure for retinoic-acid-induced transcripts in P19 embryonal carcinoma cells. Sequence analysis showed that this gene represents the first murine Wnt-8(mWnt-8) gene reported to date. The expression of the mWnt-8gene, which is rapidly induced by retinoic acid in P19 and embryonic stem cells, appears to be restricted to early stages of mouse embryogenesis, mWnt-8transcripts are first detected in the posterior region of the epiblast of early primitive streak-stage embryos. As gastrulation proceeds, mWnt-8expression spreads into the embryonic ectoderm up to a sharp rostral boundary at the base of the developing headfolds.mWnt-8is also transiently expressed in the newly formed mesoderm.mWnt-8expression is rapidly down-regulated during early somitogenesis, the latest detectable expression domains corresponding to the presumptive fourth rhombomere and the caudal region of the neural plate. The expression pattern of mWnt-8is clearly distinct from those of other murine Wntgenes expressed during gastrulation, but shows striking similarities with that of the chicken Cwnt-8Cgene. We also show that mWnt-8expression is ectopically induced in the rostral neural plate in response to RA exposure of presomitic (7ȁ7.5 days post coitum) cultured mouse embryos.

Published
1996
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