1. IgG Anti-IgE Autoantibodies in Visceral Leishmaniasis
- Author
-
Ajax Mercês Atta, M. L. B. Sousa-Atta, A. D'oliveira, Roque P. Almeida, Maria Ilma Araujo, and Edgar M. Carvalho
- Subjects
Microbiology (medical) ,lcsh:Arctic medicine. Tropical medicine ,lcsh:RC955-962 ,lcsh:QR1-502 ,Schistosomiasis ,Immunoglobulin E ,lcsh:Microbiology ,schistosomiasis mansoni ,GTP-Binding Proteins ,medicine ,Humans ,visceral leishmaniasis ,Autoantibodies ,biology ,business.industry ,Sepharose ,IgG anti-IgE ,Autoantibody ,medicine.disease ,biology.organism_classification ,Antibodies, Anti-Idiotypic ,Titer ,protein G ,Visceral leishmaniasis ,Immunoglobulin G ,Immunology ,biology.protein ,Leishmaniasis, Visceral ,Schistosoma mansoni ,Protein G ,IgE ,business ,Protein Binding ,Peroxidase - Abstract
Procedures for IgG depletion in visceral leishmaniasis (VL) and schistosomiasis sera using Sepharose-protein G beads also deplete IgE. In this study, the presence of IgG anti-IgE autoantibodies in sera from patients with VL (n = 10), and hepatic-intestinal schistosomiasis (n = 10) and from healthy individuals (n = 10) was investigated. A sandwich ELISA using goat IgG anti-human IgE to capture serum IgE and goat anti-human IgG peroxidase conjugate to demonstrate the binding of IgG to the IgE captured was performed. VL sera had higher titers (p < 0.05) of IgG anti-IgE autoantibodies (OD = 2.01 ± 0.43) than sera from healthy individuals (OD = 1.35 ± 0.16) or persons infected with Schistosoma mansoni (OD = 1.34 ± 0.18). The immunoblotting carried out with eluates from Sepharose-protein G beads used to deplete IgG from these sera and goat anti-human IgE peroxidase conjugate, showed a similar pattern of bands, predominating the 75 kDa epsilon-heavy chain and also polypeptides resulting from physiological enzymatic digestion of IgE. A frequent additional band immediately above 75 kDa was observed only in VL sera.
- Published
- 2002