1. The presence of 3-hydroxypropionate and 1,3-propanediol suggests an alternative path for conversion of glycerol to Acetyl-CoA
- Author
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Eunsook S. Jin, Craig R. Malloy, and Min H. Lee
- Subjects
3HP, 3-hydroxypropionate ,Metabolite ,GAPDH, glyceraldehyde 3-phosphate dehydrogenase ,Hamster ,ACC, acetyl-CoA carboxylase ,DHAP, dihydroxyacetone phosphate ,lcsh:Physiology ,lcsh:Biochemistry ,GK, glycerol kinase ,chemistry.chemical_compound ,PCC, propionyl-CoA carboxylase ,Ketogenesis ,Glycerol ,lcsh:QD415-436 ,α-kG, α-ketoglutarate ,Glu, glutamate ,3HPA, 3-hydroxypropionaldehyde ,lcsh:QP1-981 ,β-HB, β-hydroxybutyrate ,Acetyl-CoA ,G3P, glycerol 3-phosphate ,Gluconeogenesis ,GlyDH, glycerol dehydrogenase ,Metabolism ,Aldehyde dehydrogenase ,PDH, pyruvate dehydrogenase ,Original Research Paper ,Citric acid cycle ,ALDH, aldehyde dehydrogenase ,3-Hydroxypropionate ,chemistry ,Biochemistry ,GA3P, glyceraldehyde 3-phosphate ,1,3PDO, 1,3-propanediol ,1,3-Propanediol ,Oxidative metabolism ,OAA, oxaloacetate - Abstract
Background In our recent study using [U–13C3]glycerol, a small subset of hamsters showed an unusual profile of glycerol metabolism: negligible gluconeogenesis from glycerol plus conversion of glycerol to 1,3-propanediol (1,3PDO) and 3-hydroxypropionate (3HP) which were detected in the liver and blood. The purpose of the current study is to evaluate the association of these unusual glycerol products with other biochemical processes in the liver. Methods Fasted hamsters received acetaminophen (400 mg/kg; n = 16) or saline (n = 10) intraperitoneally. After waiting 2 h, all the animals received [U–13C3]glycerol intraperitoneally. Liver and blood were harvested 1 h after the glycerol injection for NMR analysis and gene expression assays. Results 1,3PDO and 3HP derived from [U–13C3]glycerol were detected in the liver and plasma of eight hamsters (two controls and six hamsters with acetaminophen treatment). Glycerol metabolism in the liver of these animals differed substantially from conventional metabolic pathways. [U–13C3]glycerol was metabolized to acetyl-CoA as evidenced with downstream products detected in glutamate and β-hydroxybutyrate, yet 13C labeling in pyruvate and glucose was minimal (p, Highlights • 1,3PDO and 3HP were detected in the liver of hamsters receiving [13C3]glycerol. • 1,3PDO and 3HP were associated with minimal gluconeogenesis from glycerol. • Glycerol was utilized in catabolism via acetyl-CoA with 1,3PDO and 3HP.
- Published
- 2021