1. Exploring the potential application of alternative nuclei in NMR based metabolomics.
- Author
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Sinclair GM, Oakes SM, Warden AC, Paten AM, and Jones OAH
- Subjects
- Bees, Animals, Magnetic Resonance Spectroscopy methods, Carbon, Metabolomics methods, Magnetic Resonance Imaging
- Abstract
Introduction: Nuclear magnetic resonance (NMR) is widely used in metabolomics but it focusses on
1 H over other NMR-active nuclei., Objectives: To evaluate the potential of alternative NMR-sensitive nuclei to generate useful metabolomic data., Method: Proton, carbon, phosphorus, and nitrogen-based NMR metabolomics was undertaken on extracts from mint and European honey bee tissue., Results: Carbon NMR provided useful information but required larger sample sizes. Phosphorus produced overlapping peaks in one dimensional (1D) analysis but showed potential in 2D experiments.15 N NMR was found to not be sensitive enough for general metabolomic work., Conclusions: Alternative NMR active nuclei are useful for metabolomics., (© 2023. The Author(s).)- Published
- 2023
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