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Your search keyword '"Azides pharmacokinetics"' showing total 6 results
Start Over Descriptor "Azides pharmacokinetics" Journal methods in enzymology
6 results on '"Azides pharmacokinetics"'

1. Covalent tethering of ligands to retinal rod cyclic nucleotide-gated channels: binding site structure and allosteric mechanism.

Author

Karpen JW, Ruiz M, and Brown RL

Subjects
Affinity Labels, Allosteric Regulation, Ambystoma, Animals, Azides chemical synthesis, Azides pharmacokinetics, Binding Sites, Cattle, Cyclic GMP analogs & derivatives, Cyclic GMP chemical synthesis, Cyclic GMP pharmacokinetics, Cyclic Nucleotide-Gated Cation Channels, Electrophoresis, Polyacrylamide Gel methods, Eye Proteins chemistry, Eye Proteins metabolism, Guanosine Triphosphate, Kinetics, Ligands, Peptide Fragments chemistry, Peptide Fragments isolation & purification, Phosphorus Radioisotopes, Radioisotope Dilution Technique, Rana pipiens, Ion Channels chemistry, Ion Channels metabolism, Retinal Rod Photoreceptor Cells physiology
Published
2000
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2. Identification of drug interaction sites in P-glycoprotein.

Author

Greenberger LM

Subjects
Animals, Antineoplastic Agents toxicity, Azides pharmacokinetics, Binding Sites, Cell Line, Dihydropyridines pharmacokinetics, Drug Resistance, Multiple, Epitope Mapping, Humans, Iodine Radioisotopes, Radioisotope Dilution Technique, Tritium, ATP Binding Cassette Transporter, Subfamily B, Member 1 chemistry, ATP Binding Cassette Transporter, Subfamily B, Member 1 metabolism, Affinity Labels pharmacokinetics, Antineoplastic Agents pharmacokinetics
Published
1998
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3. High-level expression of mouse Mdr3 P-glycoprotein in yeast Pichia pastoris and characterization of ATPase activity.

Author

Beaudet L, Urbatsch IL, and Gros P

Subjects
ATP Binding Cassette Transporter, Subfamily B, Member 1 isolation & purification, ATP Binding Cassette Transporter, Subfamily B, Member 1 metabolism, ATP-Binding Cassette Transporters isolation & purification, ATP-Binding Cassette Transporters metabolism, Adenosine Triphosphatases biosynthesis, Adenosine Triphosphatases isolation & purification, Affinity Labels pharmacokinetics, Animals, Azides pharmacokinetics, Binding, Competitive, Cell Fractionation methods, Cell Membrane metabolism, Cell Membrane ultrastructure, Cloning, Molecular methods, Colchicine pharmacology, Doxorubicin pharmacology, Drug Resistance, Multiple, Iodine Radioisotopes, Kinetics, Mice, Plasmids, Prazosin analogs & derivatives, Prazosin pharmacokinetics, Recombinant Proteins biosynthesis, Recombinant Proteins isolation & purification, Recombinant Proteins metabolism, Time Factors, Transformation, Genetic, Valinomycin pharmacology, Vinblastine pharmacology, ATP Binding Cassette Transporter, Subfamily B, ATP Binding Cassette Transporter, Subfamily B, Member 1 biosynthesis, ATP-Binding Cassette Transporters biosynthesis, Adenosine Triphosphatases metabolism, Pichia genetics
Published
1998
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4. Functional expression of human P-glycoprotein from plasmids using vaccinia virus-bacteriophage T7 RNA polymerase system.

Author

Hrycyna CA, Ramachandra M, Pastan I, and Gottesman MM

Subjects
ATP Binding Cassette Transporter, Subfamily B, Member 1 isolation & purification, ATP Binding Cassette Transporter, Subfamily B, Member 1 metabolism, Adenosine Triphosphatases metabolism, Affinity Labels, Azides pharmacokinetics, Cell Membrane metabolism, Cloning, Molecular methods, Colchicine pharmacokinetics, Daunorubicin pharmacokinetics, Dihydropyridines pharmacokinetics, Drug Resistance, Multiple, Electrophoresis, Polyacrylamide Gel methods, Escherichia coli, HeLa Cells, Humans, Indicators and Reagents, Iodine Radioisotopes, Osteosarcoma, Plasmids, Polymerase Chain Reaction methods, Prazosin analogs & derivatives, Prazosin pharmacokinetics, Recombinant Proteins biosynthesis, Recombinant Proteins isolation & purification, Recombinant Proteins metabolism, Restriction Mapping, Transfection methods, Tritium, Tumor Cells, Cultured, Vinblastine pharmacokinetics, Viral Proteins, ATP Binding Cassette Transporter, Subfamily B, Member 1 biosynthesis, DNA-Directed RNA Polymerases, Vaccinia virus
Published
1998
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5. Cocaine and GBR photoaffinity labels as probes of dopamine transporter structure.

Author

Vaughan RA

Subjects
Animals, Azides pharmacokinetics, Binding Sites, Carbohydrates analysis, Carrier Proteins isolation & purification, Chromatography, Affinity methods, Chromatography, Gel methods, Cocaine chemistry, Cocaine pharmacokinetics, Dopamine Plasma Membrane Transport Proteins, Glycosylation, Humans, Iodine Radioisotopes, Mutagenesis, Site-Directed, Piperazines pharmacokinetics, Radioligand Assay methods, Recombinant Proteins chemistry, Recombinant Proteins metabolism, Transfection, Affinity Labels, Azides chemistry, Brain metabolism, Carrier Proteins chemistry, Carrier Proteins metabolism, Cocaine analogs & derivatives, Membrane Glycoproteins, Membrane Transport Proteins, Nerve Tissue Proteins, Piperazines chemistry
Abstract

Several aspects of DAT structure and function have been elucidated using a combination of photoaffinity labeling, proteolysis, enzymatic deglycosylation, and epitope-specific immunoprecipitation. The two photolabels are incorporated in different regions of the protein, suggesting that the binding sites for the ligands are distinct or partially nonoverlapping, consistent with results produced by site-directed mutagenesis and analysis of chimeras. These studies have also verified several aspects of DAT structure previously hypothesized based only on theoretical considerations, including the presence of at least one transmembrane helix or other membrane-anchoring structure in two different regions of the protein, identification of the glycosylated domain, and some topological properties. It should be possible to extend and adapt these techniques to further delineate DAT structural properties and to identify other functional domains such as phosphorylation sites or active sulfhydryl moieties.

Published
1998
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6. Photoaffinity labeling of human P-glycoprotein: effect of modulator interaction and ATP hydrolysis on substrate binding.

Author

Dey S, Ramachandra M, Pastan I, Gottesman MM, and Ambudkar SV

Subjects
ATP Binding Cassette Transporter, Subfamily B, Member 1 isolation & purification, Affinity Labels pharmacokinetics, Animals, Azides pharmacokinetics, Baculoviridae, Cell Culture Techniques methods, Cell Line, Cell Membrane metabolism, Chromatography, Affinity methods, Drug Resistance, Multiple, Electrophoresis, Polyacrylamide Gel methods, Humans, Indicators and Reagents, Iodine Radioisotopes, Kinetics, Prazosin analogs & derivatives, Prazosin pharmacokinetics, Recombinant Proteins chemistry, Recombinant Proteins isolation & purification, Recombinant Proteins metabolism, Spodoptera, Transfection methods, Vanadates pharmacokinetics, Vanadates pharmacology, ATP Binding Cassette Transporter, Subfamily B, Member 1 chemistry, ATP Binding Cassette Transporter, Subfamily B, Member 1 metabolism, Adenosine Triphosphate metabolism
Published
1998
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