1. Quantification of diacylglycerol by mass spectrometry
- Author
-
Katharina, vom Dorp, Isabel, Dombrink, and Peter, Dörmann
- Subjects
Diglycerides ,Molecular Weight ,Plant Leaves ,Solid Phase Extraction ,Statistics as Topic ,Arabidopsis ,Chemical Fractionation ,Reference Standards ,Mass Spectrometry - Abstract
Diacylglycerol (DAG) is an important intermediate of lipid metabolism and a component of phospholipase C signal transduction. Quantification of DAG in plant membranes represents a challenging task because of its low abundance. DAG can be measured by direct infusion mass spectrometry (MS) on a quadrupole time-of-flight mass spectrometer after purification from the crude plant lipid extract via solid-phase extraction on silica columns. Different internal standards are employed to compensate for the dependence of the MS and MS/MS signals on the chain length and the presence of double bonds in the acyl moieties. Thus, using a combination of single MS and MS/MS experiments, quantitative results for the different molecular species of DAGs from Arabidopsis can be obtained.
- Published
- 2013