1. Plant Cell Lines in Cell Morphogenesis Research: From Phenotyping to -Omics
- Author
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Petr, Klíma, Vojtěch, Čermák, Miroslav, Srba, Karel, Müller, Jan, Petrášek, Josef, Šonka, Lukáš, Fischer, and Zdeněk, Opatrný
- Subjects
Transformation, Genetic ,Genotyping Techniques ,Gene Expression Profiling ,Tobacco ,Arabidopsis ,Cell Culture Techniques ,Cloning, Molecular ,Flow Cytometry ,Plants, Genetically Modified ,Transcriptome ,Cell Line - Abstract
Here we provide an overview of procedures for long-term cultivation, phenotyping, genotyping, and genetic transformation of cell cultures of tobacco cell lines BY-2 and VBI-0, and of A. thaliana, ecotype Landsberg erecta (LE) cell line. Notably, we present an improved protocol for BY-2 transformation and cloning and extend the available plant cell lines methodology toward high-throughput technologies like fluorescent-based cell sorting and transcriptomics.
- Published
- 2019