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Start Over Author "Saskia Neubacher" Journal methods in molecular biology (clifton, n.j.)
1 results on '"Saskia Neubacher"'

1. Detection of microRNA maturation using unmodified pre-microRNA and branched rolling circle amplification

Author

Saskia, Neubacher and Christoph, Arenz

Subjects
DNA Replication, RNA Cleavage, Ribonuclease III, MicroRNAs, Transcription, Genetic, RNA Precursors, DNA, Single-Stranded, Phosphorylation, Nucleic Acid Amplification Techniques
Abstract

The ever-increasing number of different miRNAs and their association with a vast number of cellular dysfunctions and diseases have initiated several groups to investigate miRNA maturation, which ultimately leads to down regulation of a target messenger RNA (mRNA) and its downstream product. A rapid, convenient, and reliable assay to detect the Dicer-mediated miRNA-maturation step may facilitate research in this field. Here we describe the in vitro detection of the Dicer-mediated miRNA maturation step using unmodified pre-miRNA and branched rolling circle amplification.

Published
2013

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