1. Single Cell Gene Expression Profiling of Skeletal Muscle-Derived Cells
- Author
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Sole, Gatto, Pier Lorenzo, Puri, and Barbora, Malecova
- Subjects
Quality Control ,Gene Expression Profiling ,Statistics as Topic ,Cell Culture Techniques ,Computational Biology ,Gene Expression ,Cell Separation ,Web Browser ,Flow Cytometry ,Muscle Development ,Real-Time Polymerase Chain Reaction ,Immunophenotyping ,Mice ,Animals ,Cluster Analysis ,Single-Cell Analysis ,Muscle, Skeletal ,Transcriptome ,Biomarkers ,Software - Abstract
Single cell gene expression profiling is a fundamental tool for studying the heterogeneity of a cell population by addressing the phenotypic and functional characteristics of each cell. Technological advances that have coupled microfluidic technologies with high-throughput quantitative RT-PCR analyses have enabled detailed analyses of single cells in various biological contexts. In this chapter, we describe the procedure for isolating the skeletal muscle interstitial cells termed Fibro-Adipogenic Progenitors (FAPs ) and their gene expression profiling at the single cell level. Moreover, we accompany our bench protocol with bioinformatics analysis designed to process raw data as well as to visualize single cell gene expression data. Single cell gene expression profiling is therefore a useful tool in the investigation of FAPs heterogeneity and their contribution to muscle homeostasis.
- Published
- 2017