1. Genome Editing in Penicillium chrysogenum Using Cas9 Ribonucleoprotein Particles.
- Author
-
Pohl C, Mózsik L, Driessen AJM, Bovenberg RAL, and Nygård YI
- Subjects
- Endonucleases genetics, Gene Deletion, Gene Editing methods, Gene Targeting methods, Protoplasts metabolism, Ribonucleoproteins genetics, CRISPR-Cas Systems genetics, Clustered Regularly Interspaced Short Palindromic Repeats genetics, Penicillium chrysogenum genetics, RNA, Guide, CRISPR-Cas Systems genetics
- Abstract
Several CRISPR/Cas9 tools have been recently established for precise genome editing in a wide range of filamentous fungi. This genome editing platform offers high flexibility in target selection and the possibility of introducing genetic deletions without the introduction of transgenic sequences . This chapter describes an approach for the transformation of Penicillium chrysogenum protoplasts with preassembled ribonucleoprotein particles (RNPs) consisting of purified Cas9 protein and in vitro transcribed single guide RNA (sgRNA) for the deletion of genome sequences or their replacement with alternative sequences. This method is potentially transferable to all fungal strains where protoplasts can be obtained from.
- Published
- 2018
- Full Text
- View/download PDF