1. Computational Analysis of circRNA Expression Data.
- Author
-
Ferrero G, Licheri N, De Bortoli M, Calogero RA, Beccuti M, and Cordero F
- Subjects
- Algorithms, Datasets as Topic statistics & numerical data, Humans, RNA, Circular chemistry, RNA, Circular genetics, RNA, Untranslated analysis, RNA, Untranslated chemistry, RNA, Untranslated genetics, RNA-Seq statistics & numerical data, Sequence Analysis, RNA, Software, Transcriptome, Computational Biology methods, RNA, Circular analysis, RNA-Seq methods
- Abstract
Analysis of circular RNA (circRNA) expression from RNA-Seq data can be performed with different algorithms and analysis pipelines, tools allowing the extraction of heterogeneous information on the expression of this novel class of RNAs. Computational pipelines were developed to facilitate the analysis of circRNA expression by leveraging different public tools in easy-to-use pipelines. This chapter describes the complete workflow for a computationally reproducible analysis of circRNA expression starting for a public RNA-Seq experiment. The main steps of circRNA prediction, annotation, classification, sequence reconstruction, quantification, and differential expression are illustrated.
- Published
- 2021
- Full Text
- View/download PDF