1. Mitochondrial Complexome Profiling.
- Author
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Giese H, Meisterknecht J, Heidler J, and Wittig I
- Subjects
- Cell Line, Tumor, Chromatography, Liquid methods, Humans, Membrane Proteins chemistry, Membrane Proteins metabolism, Mitochondria metabolism, Mitochondrial Membranes chemistry, Mitochondrial Membranes metabolism, Mitochondrial Proteins chemistry, Mitochondrial Proteins metabolism, Peptides chemistry, Peptides metabolism, Tandem Mass Spectrometry methods, Mass Spectrometry methods, Mitochondria chemistry, Native Polyacrylamide Gel Electrophoresis methods
- Abstract
Complexome profiling combines blue native gel electrophoresis (BNE) and quantitative mass spectrometry to define an entire protein interactome of a cell, an organelle, or a biological membrane preparation. The method allows the identification of protein assemblies with low abundance and detects dynamic processes of protein complex assembly. Applications of complexome profiling range from the determination of complex subunit compositions, assembly of single protein complexes, and supercomplexes to comprehensive differential studies between patients or disease models. This chapter describes the workflow of complexome profiling from sample preparation, mass spectrometry to data analysis with a bioinformatics tool.
- Published
- 2021
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