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This chapter compares two different techniques for monitoring photosynthetic O 2 production; the wide-spread Clark-type O 2 electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We describe how a simple membrane inlet for MIMS can be made out of a commercial Clark-type cell and outline the advantages and drawbacks of the two techniques to guide researchers in deciding which method to use. Protocols and examples are given for measuring O 2 evolution rates and for determining the number of chlorophyll molecules per active photosystem II reaction center., (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

This chapter compares two different techniques for monitoring photosynthetic O 2 production: the widespread Clark-type O 2 electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We describe how a simple membrane inlet for MIMS can be made out of a commercial Clark-type cell, and outline the advantages and drawbacks of the two techniques to guide researchers in deciding which method to use. Protocols and examples are given for measuring O 2 evolution rates and for determining the number of chlorophyll molecules per active photosystem II reaction center.