1. Prostaglandin E2 inhibits Paracoccidioides brasiliensis killing by human monocytes.
- Author
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Bordon AP, Dias-Melicio LA, Acorci MJ, Calvi SA, Serrão Peraçoli MT, and Victoriano de Campos Soares AM
- Subjects
- Adult, Cells, Cultured, Down-Regulation, Humans, Hydrogen Peroxide metabolism, Interleukin-10 biosynthesis, Interleukin-6 biosynthesis, Middle Aged, Tumor Necrosis Factor-alpha biosynthesis, Dinoprostone metabolism, Monocytes immunology, Monocytes metabolism, Paracoccidioides metabolism, Paracoccidioidomycosis immunology
- Abstract
Human monocytes lacked fungicidal activity against high virulence strain of Paracoccidioides brasiliensis, even after IFN-gamma activation. However, monocytes treated with indomethacin exhibited an effective killing against this fungus, suggesting a role of prostaglandin E2 (PGE2) in the inhibition process. Thus, the purpose of this work was to determine whether the effect of PGE2 in fungicidal activity was related with decrease on H(2)O(2) release, the metabolite involved in P. brasiliensis killing, and changes in the levels of TNF-alpha, IL-6 and IL-10. Human monocytes challenged with the fungus produced high PGE2 levels, which in turn inhibited the fungicidal activity of these cells by reducing H(2)O(2) and TNF-alpha production.
- Published
- 2007
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