1. Detection of a geographical and endemic cluster of hyper-invasive meningococcal strains
- Author
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Thierry de Baere, Corinne Ruckly, Sophie Bertrand, Jean-Marc Collard, Muhamed-Kheir Taha, Raymond Vanhoof, Eva Van Meervenne, Françoise Carion, Institut Scientifique de Santé Publique [Belgique] - Scientific Institute of Public Health [Belgium] (WIV-ISP), Réseau International des Instituts Pasteur (RIIP), Centre de Recherche Médicale et Sanitaire (Niamey, Niger) (CERMES), Infections Bactériennes Invasives, Institut Pasteur [Paris] (IP), In Belgium, this work was financed in part by the Federal Public Health Service. This publication made use of the Neisseria Multi Locus Sequence Typing website (http://pubmlst.org/neisseria/) developed by Keith Jolley and Man-Suen Chan and sited at the University of Oxford. The development of this site has been funded by the Wellcome Trust and European Union., and Institut Pasteur [Paris]
- Subjects
Male ,Serotype ,MESH: Geography ,Neisseria meningitidis, Serogroup B ,MESH: Genome, Bacterial ,MESH: Meningococcal Infections ,medicine.disease_cause ,Disease Outbreaks ,MESH: Belgium ,Belgium ,Genotype ,Cluster Analysis ,MESH: Disease Outbreaks ,MESH: Evolution, Molecular ,MESH: Aged ,Genetics ,Gel electrophoresis ,MESH: Microbial Sensitivity Tests ,0303 health sciences ,MESH: Middle Aged ,Geography ,biology ,Neisseria meningitidis ,Middle Aged ,MESH: Infant ,Electrophoresis, Gel, Pulsed-Field ,MESH: Multilocus Sequence Typing ,MESH: Electrophoresis, Gel, Pulsed-Field ,Infectious Diseases ,MESH: Young Adult ,Child, Preschool ,Female ,Neisseriaceae ,Adult ,DNA, Bacterial ,Adolescent ,Immunology ,Microbial Sensitivity Tests ,Meningococcal disease ,Microbiology ,Evolution, Molecular ,Young Adult ,03 medical and health sciences ,MESH: Neisseria meningitidis, Serogroup B ,medicine ,Humans ,Serotyping ,Aged ,Retrospective Studies ,030304 developmental biology ,MESH: Adolescent ,MESH: Humans ,030306 microbiology ,MESH: Child, Preschool ,MESH: Serotyping ,Infant ,Outbreak ,MESH: Adult ,MESH: Retrospective Studies ,medicine.disease ,biology.organism_classification ,MESH: Cluster Analysis ,MESH: DNA, Bacterial ,Virology ,MESH: Male ,Meningococcal Infections ,Multilocus sequence typing ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,MESH: Female ,Genome, Bacterial ,Multilocus Sequence Typing - Abstract
International audience; From 2006 to December 2009, 45 out of the 513 strains isolated from patients with invasive meningococcal disease in Belgium, were identified as Neisseria meningitidis serogroup B, non-serotypeable, subtype P1.14 (B:NT:P1.14). Most cases were geographically clustered in the northern part of the country. Multilocus Sequence Typing and antigen gene sequencing combined with Pulsed-Field Gel electrophoresis were used to investigate this cluster. Molecular typing showed that 39 out of these 45 N. meningitidis strains belonged to the clonal complex cc-269. The presence of the same PorA Variable Regions (VR1-VR2: 22, 14), the FetA allele (F5-1) and the highly similar Pulsed-Field Gel Electrophoresis profiles, supported genetic relatedness for 38 out of these 39 isolates. Retrospective analysis of B:NT:P1.22,14 isolates from 1999 onwards suggested that these strains belonging to the cc-269 complex, first emerged in the Belgian province of West-Flanders in 2004. This study showed that the combination of molecular tools with classical methods enabled reliable outbreak detection as well as a cluster identification.
- Published
- 2011