1. Bacterial outer membrane vesicle biogenesis: a new mechanism and its implications
- Author
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Sandro Roier, Stefan Schild, Franz G. Zingl, and Fatih Cakar
- Subjects
0301 basic medicine ,Gram-negative bacteria ,Bacterial outer membrane vesicles ,Applied Microbiology ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Microbiology ,Applied Microbiology and Biotechnology ,Bacterial cell structure ,Article ,03 medical and health sciences ,Virology ,Escherichia coli ,Genetics ,Animals ,host-pathogen interactions ,Secretion ,Vibrio cholerae ,lcsh:QH301-705.5 ,Molecular Biology ,phospholipids ,Mice, Inbred BALB C ,Organelle Biogenesis ,biology ,Chemistry ,Cytoplasmic Vesicles ,Cell Biology ,Periplasmic space ,OMV biogenesis ,vaccines ,biology.organism_classification ,gram-negative bacteria ,Haemophilus influenzae ,Cell biology ,Quorum sensing ,030104 developmental biology ,lcsh:Biology (General) ,ATP-Binding Cassette Transporters ,Female ,Parasitology ,Bacterial outer membrane ,outer membrane vesicles ,Biogenesis - Abstract
Bacterial outer membrane vesicles (OMVs) have important biological roles in pathogenesis and intercellular interactions, but a general mechanism of OMV formation is lacking. Here we show that the VacJ/Yrb ABC (ATP-binding cassette) transport system, a proposed phospholipid transporter, is involved in OMV formation. Deletion or repression of VacJ/Yrb increases OMV production in two distantly related Gram-negative bacteria, Haemophilus influenzae and Vibrio cholerae. Lipidome analyses demonstrate that OMVs from VacJ/Yrb-defective mutants in H. influenzae are enriched in phospholipids and certain fatty acids. Furthermore, we demonstrate that OMV production and regulation of the VacJ/Yrb ABC transport system respond to iron starvation. Our results suggest a new general mechanism of OMV biogenesis based on phospholipid accumulation in the outer leaflet of the outer membrane. This mechanism is highly conserved among Gram-negative bacteria, provides a means for regulation, can account for OMV formation under all growth conditions, and might have important pathophysiological roles in vivo., Bacteria release outer membrane vesicles (OMVs) that play important roles in pathogenesis and intercellular interactions. Here, Roier et al. provide evidence supporting that phospholipid accumulation in the outer leaflet of the outer membrane participates in OMV formation in Gram-negative bacteria. more...
- Published
- 2017