1. IL-10 regulate decidual Tregs apoptosis contributing to the abnormal pregnancy with Toxoplasma gondii infection.
- Author
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Lao K, Zhao M, Li Z, Liu X, Zhang H, Jiang Y, Wang Y, and Hu X
- Subjects
- Animals, Caspase 3 metabolism, Caspase 8 metabolism, Disease Models, Animal, Female, Flow Cytometry, Immunologic Factors administration & dosage, Immunologic Factors metabolism, Interleukin-10 administration & dosage, Mice, Mice, Knockout, Pregnancy, Pregnancy Complications, Infectious pathology, Pregnancy Outcome, Proteolysis, Toxoplasmosis pathology, Apoptosis, Decidua pathology, Interleukin-10 metabolism, Pregnancy Complications immunology, Pregnancy Complications, Infectious immunology, T-Lymphocytes, Regulatory drug effects, Toxoplasmosis immunology
- Abstract
This study aims to investigate whether IL-10 regulate decidual Treg cells apoptosis to reverse the abnormal pregnancy outcomes with Toxoplasma gondii (T. gondii) infection. Recombinant mouse IL-10 (rIL-10) treatment and IL-10 deficiency (IL-10(-/-)) abnormal pregnancy animal models with T. gondii infection were established. Apoptosis related molecules cleaved Caspase-3 and Caspase-8 in decidual Treg cells were examined using flow cytometry. The levels of cleaved Caspase-3 and Caspase-8 in decidual Treg cells were up-regulated with T. gondii infection. Compared to infected group, the expressions of cleaved Caspase-3 and Caspase-8 in decidual Treg cells were down-regulated in rIL-10-treated group, while up-regulated in infected IL-10(-/-) group. In addition, pregnant outcomes were improved in rIL-10-treated group, while worse in IL-10(-/-) group compared to infected group. These findings revealed that IL-10 reduced the decidual Treg cells apoptosis contributing to improving adverse pregnant outcomes following T. gondii infection., (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Published
- 2015
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