1. IL-10 regulate decidual Tregs apoptosis contributing to the abnormal pregnancy with Toxoplasma gondii infection.
- Author
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Lao, Kaixue, Zhao, Mingdong, Li, Zhidan, Liu, Xianbing, Zhang, Haixia, Jiang, Yuzhu, Wang, Yanlin, and Hu, Xuemei
- Subjects
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CELL death , *APOPTOSIS , *APOPTOTIC bodies , *PRENATAL care , *CRISIS pregnancy centers - Abstract
This study aims to investigate whether IL-10 regulate decidual Treg cells apoptosis to reverse the abnormal pregnancy outcomes with Toxoplasma gondii ( T. gondii ) infection. Recombinant mouse IL-10 (rIL-10) treatment and IL-10 deficiency (IL-10 −/− ) abnormal pregnancy animal models with T. gondii infection were established. Apoptosis related molecules cleaved Caspase-3 and Caspase-8 in decidual Treg cells were examined using flow cytometry. The levels of cleaved Caspase-3 and Caspase-8 in decidual Treg cells were up-regulated with T. gondii infection. Compared to infected group, the expressions of cleaved Caspase-3 and Caspase-8 in decidual Treg cells were down-regulated in rIL-10-treated group, while up-regulated in infected IL-10 −/− group. In addition, pregnant outcomes were improved in rIL-10-treated group, while worse in IL-10 −/− group compared to infected group. These findings revealed that IL-10 reduced the decidual Treg cells apoptosis contributing to improving adverse pregnant outcomes following T. gondii infection. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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