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[Display omitted] • A simple and highly specific ratiometric fluorescence probe for AMP was firstly developed based on Eu-MOF and carbon dots. • The sensing platform could discriminate AMP from ADP and ATP through fluorescence restortion. • The probe exhibits a broad linear range of 1.0–80.0 μmol/L with LOD of 0.25 μmol/L. The selective detection of adenosine monophosphate (AMP) is of great significance for the study of cellular energy metabolism. In this study, a ratiometric fluorescent probe was designed based on europium-based metal–organic frameworks (Eu-MOF) and nitrogen-doped carbon dots (CDs) that could specifically recognize AMP. Europium nitrate and Disodium Terephthalate (Na 2 BDC) were used to synthesize Eu-BDC with red fluorescence. The fluorescence of the Eu-BDC was effectively suppressed due to water molecules coordinating with the Eu3+. However, the addition of AMP resulted in a significant restoration of the fluorescence of Eu-BDC, while the fluorescence remained suppressed upon addition of adenosine diphosphate (ADP) and adenosine triphosphate (ATP). We developed a ratiometric fluorescence sensor that could accurately detect AMP by utilizing the blue fluorescence emitted from CDs as a reliable reference signal. Under optimal conditions, the Eu-BDC/CDs system for AMP detection exhibited a linear detection range of 1.0–80.0 μmol/L and a low limit of detection (LOD) of 0.25 μmol/L. The method displayed simple preparation, high selectivity and low cost for AMP detection. To our knowledge, this is the first highly-specify double-response biosensor for AMP. [ABSTRACT FROM AUTHOR]