1. Stability improvement of polyaniline nanocomposite immunosensor for early detection of insulin receptor antibody as biomarker of type 2 diabetes.
- Author
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Farrokhnia, Mohammadreza, Amoabediny, Ghassem, Ebrahimi, Mohammad, Ganjali, Mohammad Reza, and Arjmand, Mohammad
- Subjects
POLYANILINES ,RECEPTOR antibodies ,INSULIN antibodies ,TYPE 2 diabetes ,INSULIN receptors ,NANOCOMPOSITE materials ,BIOMARKERS - Abstract
Polyaniline nanocomposite with controllable properties was used to design and fabricate a novel electrochemical immunosensor for the early detection of type 2 diabetes. Insulin receptor antibody is a powerful predictor of type 2 diabetes development in individuals. A systematic study was carried out to investigate the effects of different polyaniline layers and the Nafion layer on the morphological, chemical, and electrochemical properties of nanocomposite immunosensor, especially the stability. The bioengineered Nafion-Au nanoparticles-polyaniline/gold electrode demonstrated outstanding electrocatalytic performance in the detection of insulin receptor antibodies with a high sensitivity (136.21 µA.ng
−1 .ml.cm−2 ) in a linear range from 0.001 to 200 ng.ml−1 as well as a low detection limit of 1.827 pg.ml−1 , response time within 10 min, remarkable selectivity, and significant stability of 80 days. Therefore, the developed immunosensor is a suitable nanocomposite platform for insulin receptor antibody level determination in human plasma. [ABSTRACT FROM AUTHOR]- Published
- 2022
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