1. Colorimetric determination of DNA using an aptamer and plasmonic nanoplatform.
- Author
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Sang, Fuming, Yin, Suyao, Pan, Jianxin, Liu, Deli, and Zhang, Zhizhou
- Subjects
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APTAMERS , *DNA , *NUCLEIC acids , *DETECTION limit , *METHYLMERCURY , *HAIRPIN (Genetics) , *THYMINE - Abstract
A facile plasmonic nanoplatform was developed for rapid and sensitive determination of nucleic acid. Hg2+-regulated molecular beacon (MB, hairpin) containing rich thymine (T) bases at both ends is used as the probe. A hairpin structure can be formed in the MB probe due to the strong binding of Hg2+ to T. However, in the presence of target DNA, the hairpin structure is opened owing to target DNA-specific hybridization with the aptamer. Simultaneously, the opened MB interacts with poly(diallyldimethylammonium chloride) (PDDA) and hinders PDDA-induced aggregation of AuNPs, accompanied by a color change from blue to red and a decrease in absorption ratio (A620/A520). Hence, a good linear relationship was observed between the decreased absorption ratio (A620/A520) and DNA concentration ranging from 0.02 to 2 nmol/L with a low detection limit of 4.42 pmol/L. Moreover, this nanoplatform has been successfully utilized to discriminate between perfect target and mismatch sequences. More importantly, the bioassay is simple, versatile, rapid, and cost-effective compared with other common methods, which holds great promise for clinical diagnosis and biomedical application. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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