1. Lysosome-targetable selenium-doped carbon nanodots for in situ scavenging free radicals in living cells and mice
- Author
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Hong Huang, Zuming Hu, Danling Zhou, and Junrong Yu
- Subjects
Antioxidant ,Free Radicals ,Biocompatibility ,Radical ,medicine.medical_treatment ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Analytical Chemistry ,Mice ,Selenium ,chemistry.chemical_compound ,Morpholine ,Lysosome ,Quantum Dots ,medicine ,Animals ,Humans ,Moiety ,Chemistry ,021001 nanoscience & nanotechnology ,Carbon ,0104 chemical sciences ,medicine.anatomical_structure ,Biophysics ,Phorbol ,Surface modification ,lipids (amino acids, peptides, and proteins) ,Lysosomes ,0210 nano-technology - Abstract
Lysosome-targetable selenium-doped carbon nanodots (Lyso-Se-CDs) that can efficiently scavenge lysosomal •OH in living cells and mice were designed in this research. Se-CDs with redox-responsive fluorescence (λex = 379 nm, λem = 471 nm, quantum yield = 7.1%) were initially synthesized from selenocystine by a facile hydrothermal method, followed by the surface modification with morpholine, a lysosome targeting moiety. The as-synthesized Lyso-Se-CDs exhibited excellent colloidal stability, efficient scavenging abilities towards •OH, low biotoxicity, as well as good biocompatibility and lysosome targetability. Due to these desirable properties, Lyso-Se-CDs had been successfully utilized for rescuing cells from elevated lysosomal •OH levels. More importantly, Lyso-Se-CDs efficiently relieved phorbol 12-myristate 13-acetate (PMA) triggered ear inflammation in live mice. These findings reveal that Lyso-Se-CDs are potent candidates for treating •OH-related inflammation.
- Published
- 2021
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