1. Apical-basal polarity of the spectrin cytoskeleton in the C. elegans vulva.
- Author
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Barker TJ, Chan FY, Carvalho AX, and Sundaram MV
- Abstract
The C. elegans vulva is a polarized epithelial tube that has been studied extensively as a model for cell-cell signaling, cell fate specification, and tubulogenesis. Here we used endogenous fusions to show that the spectrin cytoskeleton is polarized in this organ, with conventional beta-spectrin ( UNC-70 ) found only at basolateral membranes and beta heavy spectrin ( SMA-1 ) found only at apical membranes. The sole alpha-spectrin ( SPC-1 ) is present at both locations but requires SMA-1 for its apical localization. Thus, beta spectrins are excellent markers for vulva cell membranes and polarity., Competing Interests: The authors declare that there are no conflicts of interest present., (Copyright: © 2023 by the authors.)
- Published
- 2023
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