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N-doped carbon quantum dots (N-CDs) with polyaminobenzene hydrazine as precursor were prepared by solvothermal method for the monitoring of pH fluctuation in HeLa cells via fluorescence imaging. The N-CDs show two emission wavelengths at 582 and 640 nm under different pH with two excitation wavelengths. The fluorescence intensity at 640 nm (λ ex  = 520 nm) and the ratio of F 582 /F 640 (λ ex  = 470 nm) linearly increase with pH in the range of 2.4 ~ 3.6 (R 2  = 992) and 5.6 ~ 7.6 (R 2  = 0.987), respectively. The sensor exhibits high sensitivity and reversibility and anti-interference capability, thus enabling sensing pH change in intracellular environment in real time, as demonstrated by successful monitoring of intracellular pH fluctuation during H 2 O 2 stimulation in HeLa cells., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

A label-free and specific FRET-based interleukin-6 (IL-6) aptasensor was developed using a DNA aptamer modified with nitrogen-doped carbon quantum dots (NCDs) and gold nanoparticles (AuNPs) as a donor-quencher pair. The assayed target was capable of disrupting the donor-acceptor assemblies yielding a concentration-related fluorescence recovery of NCDs (λ em  = 445 nm and λ ex  = 350 nm). By designing two different probes, the interaction of DNA aptamers with IL-6 protein was studied using FRET efficiency. It appeared that the sensing probes showed slightly different sensing profiles. One of the aptasensors showed a linear response of 1.5-5.9 pg/mL for IL-6 with a coefficient of determination of R 2  ≥ 0.99 and the a detection limit of 0.82 pg/mL (at S/N = 3). The experimental results indicated that the biosensor can be applied to determine IL-6 in human serum (with recovery of 95.7-102.9%). Due to the high sensitivity, excellent selectivity, and simplicity of the procedure, this strategy represents a promising alternative for IL-6 sensing in clinical applications., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

A new fluorescence method was established for sensitive detection of β-galactosidase (β-gal) activity in spiked human serum and screening of inhibitor. Nitrogen-doped carbon quantum dots (N-CQDs) were prepared by solvothermal polymerization of N-methyl-2-pyrrolidinone in an alkaline condition. The colloidal N-CQDs exhibit good water solubility, stability, and emit bright green fluorescence with a maximum emission peak at 528 nm upon excitation at 420 nm. β-gal specifically catalyzes the decomposition of its substrate P-nitrophenyl-β-D-galactopyranoside into 4-nitrophenol, whose absorption spectrum overlaps well with the excitation spectrum of the N-CQDs. As a result, the fluorescence of the N-CQDs is remarkably quenched by 4-nitrophenol via an inner filter effect. The sensing platform presents a linear response range for β-gal activity from 0.05 to 3.0 U·L -1 with a low limit of detection of 0.023 U·L -1 . An acceptable precision is obtained with a relative standard deviation (RSD) of 3.1% for 1.0 U·L -1 β-gal (n = 11). The method was applied to determine β-gal in spiked human serums with recoveries in the range  96.3-104.7%. The method was employed to evaluate inhibitor screening with D-galactal and chloroquine diphosphate as models., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

A novel photoelectrochemical (PEC) immunosensor for the determination of cardiac troponin I (cTnI) was constructed. The flower-like stannic oxide (SnO 2 ) with large specific surface area was prepared by hydrothermal synthesis. Nitrogen-doped carbon quantum dots (NCQDs) with excellent surface property were used as a sensitizer for SnO 2 . Bismuth oxyiodide (BiOI) is a narrow band gap (1.83 eV) nanomaterial, which was firstly modified on NCQDs-sensitized SnO 2 through in situ growth method. After NCQDs with small size and BiOI nanoparticles are successively combined with SnO 2 , the SnO 2 /NCQDs/BiOI microflower was obtained, which possessed good photochemical properties. Using visible light as excitation source and ascorbic acid (AA) as electron donor, the ultrasensitive and quantitative determination of cTnI was realized by detecting the changes of photocurrent under different concentrations of cTnI. The PEC immunosensor showed a large-scaled response (0.001-100 ng mL -1 ) and a low detection limit (0.3 pg mL -1 ) under optimised experimental conditions. The sensor has potential clinical value in the prediction and diagnosis of cardiovascular diseases in elderly patients with diabetes. Graphical abstract.

A fluorometric assay is described for doxycycline detection. It is based on the use of nitrogen-doped carbon quantum dots (NCQDs) coated with molecularly imprinted polymers (MIPs). The NCQDs were prepared by a one-step hydrothermal reaction using citric acid and ethylenediamine (EDA) as the starting materials. Afterwards, the NCQDs were incorporated into the polymer that was molecularly imprinted with doxycycline. It is found that doxycycline quenches the fluorescence of the NCQDs, and that the functional groups on the surface of NCQDs play an important role in terms of quenching efficiency. A larger fraction of carboxyl groups presented on the surface of NCQDs leads to a higher quenching efficiency due to the enhanced electron transfer from NCQD to doxycycline. The NCQDs@MIP composite can specifically and rapidly recognize doxycycline. Fluorescence drops linearly in the 5 to 50 μM doxycycline concentration range, and the limit of detection is 87 nM. This method was successfully applied to the determination of doxycycline in spiked pig serum where it gave recovery rates of >94%. Graphical abstract Schematic illustration for fabricating a fluorescent sensor based on nitrogen-doped carbon quantum dots (NCQDs) and molecularly imprinted polymers (MIPs). The sensor integrates the merits of the high sensitivity of NCQD and good selectivity of MIPs, and can be significantly quenched upon interaction with doxycycline.