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Ruby (red corundum) is one of the most prominent colored gemstones in the world. The highest-quality ruby ("pigeon blood" ruby) comes from marbles of the Mogok Stone Tract in central Myanmar. Although Mogok ruby has been exploited since the 6th century AD, the formation time of this gemstone is ambiguous and controversial. In this paper, we describe a mineralogical, geochemical, and geochronological study of ruby and titanite in ruby-bearing marbles obtained from an outcrop in the Mogok Stone Tract, central Myanmar. Petrographic observations have shown that titanite generally occurs in the marble matrix or occurs as inclusions in ruby. These two types of titanite exhibit identical chemical compositions. In situ secondary ion mass spectrometer (SIMS) U–Pb dating of the separated titanite from two representative samples of ruby-bearing marbles yielded lower intercept ages of 25.15 ± 0.24 Ma (MSWD = 0.26) and 25.06 ± 0.22 Ma (MSWD = 0.15), respectively. Because the closure temperature of the U–Pb system in titanite is close to the temperature of ruby growth, the obtained U–Pb ages (~25 Ma) are suggested to represent the timing of the studied ruby formation in Mogok. The acquired ages are in agreement with the timing of post-collisional extension in the Himalaya related to the migration of the eastern Himalayan syntaxis. Combining our dating results with previous geochronological data from the Mogok Stone Tract, we suggest that the formation of the studied ruby is most likely related to the high-temperature metamorphic event in the marbles during the India–Asia collision. Our study not only confirms that texturally constrained titanite could be a precise geochronometer to date the mineralization of different types of ruby, but also provides important geochronological information linking gemstone formation to the India–Asia collision. [ABSTRACT FROM AUTHOR]