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We evaluated genetic differentiation among ten presumed Japanese threadfin bream,Nemipterus japonicuspopulations along the coast of Peninsular Malaysia based on the partial sequence of the mitochondrial cytochromebgene (982 bp). Genetic divergences (Kimura-2 parameter) ranged from 0.5% to 0.8% among nine of the ten populations while these nine populations were 4.4% to 4.6% diverged from the Kuala Besar population located at the Northeast coast. The constructed Neighbour Joining (NJ) phylogenetic trees based on haplotypes showed the Kuala Besar population forming an isolated cluster. The Analysis of Molecular Variance (AMOVA) of the ten populationsa prioriassigned into four regions, revealed that most of the variation occurred within population with a fairly low but significant level of regional differentiation (FST = 0.07,p < 0.05, FSC = 0.00,p > 0.05 and FCT = 0.07,p < 0.05) attributed to the Kuala Besar population.pValue after Bonferroni correction revealed that only pairwise FSTvalues involving the Kuala Besar population with the other nine populations were significant. Thus, this study revealed that theN. japonicuspopulations off Peninsular Malaysia were panmictic. However, the Kuala Besar population, although morphologically identical was composed of a genetically discrete taxon from the rest. These findings are important contributions in formulating sustainable fishery management policies for this important fishery in Peninsular Malaysia. [ABSTRACT FROM AUTHOR]

The complete mitochondrial sequence of the Japanese threadfin bream,Nemipterus japonicushas been determined. The circle genome is 16,995 bp in size, and consists of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region. The gene order and composition ofN. japonicuswas similar to that of most other teleosts. The base composition of H-strand is 28.11% (A), 28.02% (T), 16.64 % (G) and 27.24 % (C), with an AT content of 56.12%. All genes are encoded on the heavy strand with the exception ofND6and eight tRNA genes. The mitochondrial genome ofN. japonicuspresented will be in favor of resolving phylogenetic relationships within the family Nemipteridae and the Perciformes. [ABSTRACT FROM AUTHOR]