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The genus Paphiopedilum is well known as the lady's slipper orchid in Orchidaceae family . Paphiopedilum bellatulum (Rchb.f.) Stein 1892, has important medicinal and ornamental value, which occurs in the tropical Asia. However, in recent decades, it was threatened with extinction by significantly reduced small population size. In this study, we sequenced and characterized the complete chloroplast genome of P. bellatulum based on the Illumina Hiseq platform. The size of P. bellatulum chloroplast genome was 156,567 bp, including a large single-copy (LSC) region of 88,243 bp, a small single-copy (SSC) region of 3652 bp, and two inverted repeat regions (IRs) of 32,336 bp. The overall GC contents of the chloroplast genome were 35.71%. A total of 122 genes were annotated, including 76 protein-coding genes, 38 transfer RNAs (tRNAs), and eight ribosomal RNAs (rRNAs). The phylogenetic analysis indicated that P. bellatulum formed a close relationship with another Paphiopedilum species P. wenshanense . The results will provide helpful genetic resource for further phylogenetic studies of the genus Paphiopedilum ., Competing Interests: No potential conflict of interest was reported by the authors., (© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)

Paphiopedilum henryanum Braem 1987 is a critically Endangered terrestrial orchid mainly occurred around the southern China and northern Vietnamese borders. Recently, the population size of this species has been sharply declined due to many threats such as climate change, habitat loss, and narrow distribution. In this study, the complete chloroplast genome sequence of P. henryanum was determined from Illumina pair-end sequencing data. The chloroplast genome was 155,886 bp in length, including a large single copy region (LSC) of 87,573 bp, a small single-copy region (SSC) of 2,831 bp, and a pair of inverted repeated regions (IRa and IRb) of 32,741 bp each. The chloroplast genome contained 121 genes corresponding to 76 protein-coding genes, 8 ribosomal RNA (rRNA) genes and 37 transfer RNA (tRNA) genes, respectively. The results of phylogenetic analysis indicated that P. henryanum was closely related to P. violascens and P. venustum in the genus Paphiopedilum based on sixteen whole chloroplast genome sequences. The results would provide a valuable resource for future genetic studies of Paphiopedilum ., Competing Interests: No potential conflict of interest was reported by the author(s)., (© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)

Paphiopedilum 'GZSLKY Youyou'. is a new cultivar of Paphiopedilum with highly ornamental and horticultural value developed by crossing female parent Paphiopedilum dianthum T. Tang & F. T. Wang 1940 and male parent Paphiopedilum barbigerum T. Tang & F. T. Wang 1940. In this study, the complete chloroplast genome of the cultivar has been reconstructed from the Illumina sequencing data. The circular genome was 160,503 bp in size, containing a large single-copy region (91,582 bp), a small single-copy region (3,215 bp) and a pair of IR regions (each one 32,853 bp). The overall GC contents of the chloroplast genome was 36.20%. The chloroplast genome contained 122 genes, including 76 protein coding genes, 38 tRNA genes and 8 rRNA genes. A maximum likelihood (ML) phylogenetic tree showed that the cultivar 'GZSLKY Youyou' was clustered into the same clade with its parents and was closest related to P. dianthum , reflecting a maternal inheritance of chloroplasts. This complete chloroplast genome resource could be further used for genomic studies, phylogenetic analyses, and genetic engineering and breeding of the genus Paphiopedilum ., Competing Interests: No potential conflict of interest was reported by the author(s)., (© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)