1. Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria
- Author
-
Dujeancourt, Laurent, Richter, Ricarda, Chrzanowska-Lightowlers, Zofia M., Bonnefoy, Nathalie, and Herbert, Christopher J.
- Subjects
Terminator Regions, Genetic ,Translation ,Sequence Homology, Amino Acid ,Mitochondrial ribosome tagging ,Immunoblotting ,Molecular Sequence Data ,Cell Biology ,Fission yeast ,Article ,Mitochondria ,Release factor ,Schizosaccharomyces ,Molecular Medicine ,Peptidyl tRNA hydrolase ,Amino Acid Sequence ,Schizosaccharomyces pombe Proteins ,Molecular Biology ,Carboxylic Ester Hydrolases ,Gene Deletion - Abstract
Mitochondrial translation synthesizes key subunits of the respiratory complexes. In Schizosaccharomyces pombe, strains lacking Mrf1, the mitochondrial stop codon recognition factor, are viable, suggesting that other factors can play a role in translation termination. S. pombe contains four predicted peptidyl tRNA hydrolases, two of which (Pth3 and Pth4), have a GGQ motif that is conserved in class I release factors. We show that high dosage of Pth4 can compensate for the absence of Mrf1 and loss of Pth4 exacerbates the lack of Mrf1. Also Pth4 is a component of the mitochondrial ribosome, suggesting that it could help recycling stalled ribosomes., Highlights • In S. pombe the peptidyl tRNA hydrolases Pth3 and Pth4 are mitochondrial proteins. • Pth3 and Pth4 are associated with the mitochondrial ribosome and the large subunit. • Deletion of pth4 and mrf1, encoding the mitochondrial release factor, is co-lethal. • Over-expression of pth4 compensates for the deletion of mrf1. • Pth4 can act as a release factor in S. pombe mitochondria.
- Published
- 2013