1. Development of microsatellite loci in zoonotic tapeworm Dibothriocephalus latus (Linnaeus, 1758), Lühe, 1899 (syn. Diphyllobothrium latum) using microsatellite library screening.
- Author
-
Bazsalovicsová, Eva, Koleničová, Alžbeta, Králová-Hromadová, Ivica, Minárik, Gabriel, Šoltys, Katarína, Kuchta, Roman, and Štefka, Jan
- Subjects
- *
MICROSATELLITE repeats , *TAPEWORMS , *FOODBORNE diseases , *NUCLEOTIDE sequencing , *HARDY-Weinberg formula , *POLYMERASE chain reaction - Abstract
Graphical abstract Highlights • Microsatellite markers designed for Dibothriocephalus latus. • 122 candidate loci selected after microsatellite library screening using NGS. • 78 loci approved after PCR tests and Sanger sequencing. • 6 loci for future application in population genetic studies of D. latus. Abstract The broad fish tapeworm Dibothriocephalus latus is a causative agent of human food-borne disease called diphyllobothriosis. Medical importance, scattered geographical distribution and unknown origin of D. latus in Europe and North America make this species to be an interesting model for population genetics. Microsatellite markers were originally designed by library screening using NGS approach and validated as tools for future studies on population genetics of D. latus. Out of 122 candidates selected after NGS analysis, 110 yielded PCR products of the expected size, and in 78 of them, a declared repetitive motif was confirmed by Sanger sequencing. After the fragment analysis, six loci were proved to be polymorphic and tested for observed (Ho) and expected (He) heterozygosity, and deviations from Hardy-Weinberg equilibrium (HWE). They promise future application in studies on genetic interrelationships, origin and migratory routes of this medically important emerging tapeworm. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF