1. Interleukin-1 system in the human fallopian tube-No spatial but a temporal regulation of mRNA and protein expression
- Author
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A. Schanz, D.M. Baston-Buest, J.S. Kruessel, P. Bielfeld, Jens Hirchenhain, and Alexandra P. Hess
- Subjects
medicine.medical_specialty ,Time Factors ,Interleukin-1beta ,Biology ,Biochemistry ,Andrology ,03 medical and health sciences ,Interleukin-1 receptor type I (IL-1R tI) ,0302 clinical medicine ,Endocrinology ,Interleukin-1 (IL-1) system ,In vivo ,Pregnancy ,Internal medicine ,medicine ,Humans ,RNA, Messenger ,Molecular Biology ,Gene ,Fallopian Tubes ,Menstrual Cycle ,030304 developmental biology ,0303 health sciences ,Messenger RNA ,030219 obstetrics & reproductive medicine ,Embryogenesis ,Interleukin ,Embryo ,Spatial and temporal expression pattern ,Implantation ,Hormones ,Pregnancy, Ectopic ,Postmenopause ,Interleukin 1 Receptor Antagonist Protein ,medicine.anatomical_structure ,Gene Expression Regulation ,Human fallopian tube ,Female ,Interleukin-1 receptor antagonist (IL-1ra) ,Fallopian tube ,Hormone ,Interleukin-1 - Abstract
International audience; The human fallopian tube provides the environment for the first 5 days of embryonic development . The IL-1 system is involved in human embryo implantation. This study aimed to investigate IL-1β, IL-1ra and IL-1R tI expression within the length of the human fallopian tube on mRNA- and protein-level in samples from proliferative versus secretory phase, postmenopause samples (PMP) and samples from intra- (IUP) and extrauterine (EUP) pregnancies to examine possible spatial and hormonal induced changes (fimbrial, ampullary and isthmic tube segments). On mRNA-level, IL-1β was expressed in all samples except in PMP. IL-1R tI could be detected in all samples whereas IL-1ra was only expressed in secretory phase and the IUP sample. Immunohistochemically we could detect IL-1β and IL-1R t1 protein in all proliferative and secretory phase samples with maximum intensity in secretory phase samples whereas IL-1ra was expressed in secretory phase samples only. Overall no spatial but temporal differences possibly due to hormonal changes could be observed suggesting a precise regulation of the IL-1 system, especially for IL-1ra and moreover a stable molecular architecture within the full length of the fallopian tube.
- Published
- 2007
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