1. Triiodothyronine activated extranuclear pathways upregulate adiponectin and leptin in murine adipocytes.
- Author
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Mathias LS, Rodrigues BM, Gonçalves BM, Moretto FCF, Olimpio RMC, Deprá I, De Sibio MT, Tilli HP, Nogueira CR, and de Oliveira M
- Subjects
- 3T3-L1 Cells, Adipocytes metabolism, Animals, Cell Differentiation drug effects, Cell Differentiation genetics, Cytoplasm drug effects, Cytoplasm metabolism, Gene Expression drug effects, Mice, Signal Transduction drug effects, Thyroxine analogs & derivatives, Thyroxine pharmacology, Up-Regulation drug effects, Adipocytes drug effects, Adiponectin metabolism, Leptin metabolism, Triiodothyronine pharmacology
- Abstract
Adiponectin and leptin, important for metabolic regulation, are synthesized and secreted by adipose tissue and are influenced by triiodothyronine (T3) that activates the MAPK/ERK and integrin αVβ3 pathways, modulating gene expression. Adipocytes were treated with T3 (10 nM), for 1 h, in the absence or presence of PD98059 (PD) and tetraiodothyroacetic acid (Tetrac), which are pathways inhibitors. The cells were incubated with Adipo Red/Oil Red O reagents, and intracellular lipid accumulation [glycerol and triacylglycerol (TAG)], MTT, 8-hydroxideoxyguanosine (8-OH-dG), and mRNA and protein expression were assessed. T3 increased leptin mRNA and protein expression, and, in contrast, there was a decrease in the Tetrac + T3 group. Adiponectin mRNA expression was not altered by T3, though it had increased its protein expression, which was terminated by inhibitors PD + T3 and Tetrac + T3. However, T3 did not alter PPARγ protein expression, lipid accumulation, TAG, glycerol, and DNA damage, but PD + T3 and Tetrac + T3 reduced these parameters. T3 activated the MAPK/ERK pathway on adipocytes to modulate the adiponectin protein expression and integrin αvβ3 to alter the leptin gene expression., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2020
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