1. Presence of c-kit mRNA in goat ovaries and improvement of in vitro preantral follicle survival and development with kit ligand.
- Author
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Lima IM, Brito IR, Rodrigues GQ, Silva CM, Magalhães-Padilha DM, Lima LF, Celestino JJ, Campello CC, Silva JR, Figueiredo JR, and Rodrigues AP
- Subjects
- Animals, Cattle, Cell Proliferation drug effects, Cells, Cultured, Culture Media pharmacology, Female, Fluorescence, Follicle Stimulating Hormone pharmacology, Humans, Meiosis drug effects, Oocytes cytology, Oocytes drug effects, Oocytes metabolism, Ovarian Follicle drug effects, Ovarian Follicle metabolism, Proto-Oncogene Proteins c-kit metabolism, RNA, Messenger genetics, Tissue Culture Techniques, Gene Expression Regulation, Developmental drug effects, Goats genetics, Goats growth & development, Ovarian Follicle growth & development, Proto-Oncogene Proteins c-kit genetics, Stem Cell Factor pharmacology, Tissue Survival drug effects
- Abstract
This study evaluated the levels of c-kit mRNA in goat follicles and the effects of kit ligand (KL) on the in vitro development of cultured preantral follicles. Preantral follicles isolated from goat ovarian cortex were cultured for 18 days in α-MEM(+) supplemented with KL (0, 50 or 100 ng/mL) in the absence or presence of follicle stimulating hormone (FSH). Real-time PCR showed that c-kit mRNA was higher in primordial and primary follicles than in secondary stage. Regarding the culture, KL addition in the absence of FSH improved the follicular survival, antrum formation, oocyte growth and meiotic resumption. KL-positive effects were not observed in the presence of FSH. In conclusion, c-kit mRNAs are detected in all follicular categories. KL promotes the survival and antral cavity formation of caprine preantral follicles after in vitro culture, as well as the growth and meiotic resumption of their oocytes in the absence of FSH., (Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2011
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