1. Oligodendrocyte differentiation and myelination defects in OMgp null mice
- Author
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Mengsheng Qiu, Yinghui Hu, Xinhua Lee, Zhongshu Yang, Zhaohui Shao, Pepinsky R Blake, Sha Mi, Yi Ping Zhang, and Robert H. Miller
- Subjects
Null mice ,Encephalomyelitis, Autoimmune, Experimental ,Neural Conduction ,Biology ,GPI-Linked Proteins ,Nerve conduction velocity ,Cellular and Molecular Neuroscience ,Mice ,In vivo ,medicine ,Animals ,Humans ,Molecular Biology ,Cells, Cultured ,Myelin Sheath ,Mice, Knockout ,Myelination defects ,Oligodendrocyte differentiation ,Cell Differentiation ,Cell Biology ,Clinical disease ,Oligodendrocyte ,In vitro ,Cell biology ,Mice, Inbred C57BL ,Myelin-Associated Glycoprotein ,Oligodendroglia ,medicine.anatomical_structure ,Myelin-Oligodendrocyte Glycoprotein ,Neuroscience ,Myelin Proteins - Abstract
OMgp is selectively expressed in CNS by oligodendrocyte. However, its potential role(s) in oligodendrocyte development and myelination remain unclear. We show that OMgp null mice are hypomyelinated in their spinal cords, resulting in slower ascending and descending conduction velocities compared to wild-type mice. Consistent with the hypomyelination, in the MOG induced EAE model, OMgp null mice show a more severe EAE clinical disease and slower nerve conduction velocity compared to WT animals. The contribution of OMgp to oligodendrocyte differentiation and myelination was verified using cultured oligodendrocytes from null mice. Oligodendrocytes isolated from OMgp null mice show a significant decrease in the number of MBP+ cells and in myelination compared to wild-type mice. The dramatic effects of the OMgp KO in oligodendrocyte maturation in vivo and in vitro reveal a new and important function for OMgp in regulating CNS myelination.
- Published
- 2011