1. Utilization of recombinase polymerase amplification method combined with lateral flow dipstick for visual detection of respiratory syncytial virus
- Author
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Du-zhi Fang, Qin-fei Zhao, Ming-gang Cheng, Chao-ming Cai, Fang-Fang Chen, and Yu-Zhong Xu
- Subjects
Recombinase Polymerase Amplification ,Biology ,Real-Time Polymerase Chain Reaction ,Sensitivity and Specificity ,Virus ,Recombinases ,03 medical and health sciences ,medicine ,Humans ,Respiratory system ,Molecular Biology ,Gene ,030304 developmental biology ,0303 health sciences ,030306 microbiology ,Reproducibility of Results ,food and beverages ,Cell Biology ,Dipstick ,Virology ,Reverse transcriptase ,Visual detection ,medicine.anatomical_structure ,Respiratory Syncytial Virus, Human ,Rheology ,Respiratory tract - Abstract
Respiratory syncytial virus (RSV) is a major causative agent of respiratory tract infection necessitating hospitalization in children. A rapid diagnostic method would facilitate early detection of RSV infection and timely implementation of special treatment. Here, a reverse transcription recombinase polymerase amplification (RT-RPA) assay combined with lateral flow dipstick (LFD) was evaluated for rapid visual detection of RSV. The primers were designed to target the conserved L gene. The RT-RPA-LFD assay could simultaneously detect RSV subtype A and B with the same detection limit of 10 copies of a given RNA molecule. Moreover, the assay showed no cross-reactivity with other common human pathogens. The performance of the RT-RPA-LFD assay was evaluated by testing 136 nasopharyngeal aspirates (NPAs). The agreement of the detection results between RT-RPA-LFD and qRT-PCR was 100% (34 positive and 102 negative cases). In summary, the developed RT-RPA-LFD assay had good performance in detecting RSV in clinical specimens, thus providing a novel alternative solution for the detection of RSV under field conditions.
- Published
- 2020