1. Telomeric DNA Mediates De Novo PML Body Formation
- Author
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Anneke K. Brouwer, Joost Schimmel, Alfred C.O. Vertegaal, Joop Wiegant, Hans J. Tanke, and Roeland W. Dirks
- Subjects
Recombinant Fusion Proteins ,Promyelocytic Leukemia Protein ,Biology ,Inclusion bodies ,Mice ,chemistry.chemical_compound ,Leukemia, Promyelocytic, Acute ,Small Ubiquitin-Related Modifier Proteins ,medicine ,Animals ,Humans ,Telomeric Repeat Binding Protein 2 ,Telomeric Repeat Binding Protein 1 ,Nuclear protein ,Molecular Biology ,Transcription factor ,Cells, Cultured ,Inclusion Bodies ,Mice, Knockout ,Tumor Suppressor Proteins ,Nuclear Proteins ,Articles ,DNA ,Cell Biology ,Telomere ,Molecular biology ,Cell nucleus ,medicine.anatomical_structure ,chemistry ,Transcription Factors - Abstract
The cell nucleus harbors a variety of different bodies that vary in number, composition, and size. Although these bodies coordinate important nuclear processes, little is known about how they are formed. Among the most intensively studied bodies in recent years is the PML body. These bodies have been implicated in gene regulation and other cellular processes and are disrupted in cells from patients suffering from acute promyelocytic leukemia. Using live cell imaging microscopy and immunofluorescence, we show in several cell types that PML bodies are formed at telomeric DNA during interphase. Recent studies revealed that both SUMO modification sites and SUMO interaction motifs in the promyelocytic leukemia (PML) protein are required for PML body formation. We show that SMC5, a component of the SUMO ligase MMS21-containing SMC5/6 complex, localizes temporarily at telomeric DNA during PML body formation, suggesting a possible role for SUMO in the formation of PML bodies at telomeric DNA. Our data identify a novel role of telomeric DNA during PML body formation.
- Published
- 2009
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