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Your search keyword '"Yates, John R."' showing total 23 results
Start Over Author "Yates, John R." Journal molecular biology of the cell
23 results on '"Yates, John R."'

10. Biological and Structural Basis for Aha1 Regulation of Hsp90 ATPase Activity in Maintaining Proteostasis in the Human Disease Cystic Fibrosis

Author

Koulov, Atanas V., primary, LaPointe, Paul, additional, Lu, Bingwen, additional, Razvi, Abbas, additional, Coppinger, Judith, additional, Dong, Meng-Qiu, additional, Matteson, Jeanne, additional, Laister, Rob, additional, Arrowsmith, Cheryl, additional, Yates, John R., additional, and Balch, William E., additional

Published
2010
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21. Multiple inputs control sulfur-containing amino acid synthesis in Saccharomyces cerevisiae

Author

Sadhu, Meru J., Moresco, James J., Zimmer, Anjali D., Yates, John R., and Rine, Jasper

Abstract

Genes in Saccharomyces cerevisiaeinvolved in sulfur-containing amino acid synthesis are transcriptionally induced by either cysteine or S-adenosyl-methionine deficiency, as well as defects in phosphatidylcholine synthesis. Met30p, a regulator of these genes, changes physically in inducing conditions, which may mediate its regulatory activity.

Published
2014
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22. Nbl1p: A Borealin/Dasra/CSC-1-like Protein Essential for Aurora/Ipl1 Complex Function and Integrity in Saccharomyces cerevisiae

Author

Nakajima, Yuko, Tyers, Randall G., Wong, Catherine C.L., Yates, John R., Drubin, David G., and Barnes, Georjana

Abstract

The Aurora kinase complex, also called the chromosomal passenger complex (CPC), is essential for faithful chromosome segregation and completion of cell division. In Fungi and Animalia, this complex consists of the kinase Aurora B/AIR-2/Ipl1p, INCENP/ICP-1/Sli15p, and Survivin/BIR-1/Bir1p. A fourth subunit, Borealin/Dasra/CSC-1, is required for CPC targeting to centromeres and central spindles and has only been found in Animalia. Here we identified a new core component of the CPC in budding yeast, Nbl1p. NBL1is essential for viability and nbl1mutations cause chromosome missegregation and lagging chromosomes. Nbl1p colocalizes and copurifies with the CPC, and it is essential for CPC localization, stability, integrity, and function. Nbl1p is related to the N-terminus of Borealin/Dasra/CSC-1 and is similarly involved in connecting the other CPC subunits. Distant homology searching identified nearly 200, mostly unannotated, Borealin/Dasra/CSC-1–related proteins from nearly 150 species within Fungi and Animalia. Analysis of the sequence of these proteins, combined with comparative protein structure modeling of Bir1p-Nbl1p-Sli15p using the crystal structure of the human Survivin–Borealin–INCENP complex, revealed a striking structural conservation across a broad range of species. Our biological and computational analyses therefore establish that the fundamental design of the CPC is conserved from Fungi to Animalia.

Published
2009
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23. Cip1 and Cip2 Are Novel RNA-Recognition-Motif Proteins That Counteract Csx1 Function during Oxidative Stress

Author

Martín, Victoria, Rodríguez-Gabriel, Miguel A., McDonald, W. Hayes, Watt, Stephen, Yates, John R., Bähler, Jürg, and Russell, Paul

Abstract

Eukaryotic cells reprogram their global patterns of gene expression in response to stress. Recent studies in Schizosaccharomyces pombeshowed that the RNA-binding protein Csx1 plays a central role in controlling gene expression during oxidative stress. It does so by stabilizing atf1+mRNA, which encodes a subunit of a bZIP transcription factor required for gene expression during oxidative stress. Here, we describe two related proteins, Cip1 and Cip2, that were identified by multidimensional protein identification technology (MudPIT) as proteins that coprecipitate with Csx1. Cip1 and Cip2 are cytoplasmic proteins that have RNA recognition motifs (RRMs). Neither protein is essential for viability, but a cip1Δ cip2Δ strain grows poorly and has altered cellular morphology. Genetic epistasis studies and whole genome expression profiling show that Cip1 and Cip2 exert posttranscriptional control of gene expression in a manner that is counteracted by Csx1. Notably, the sensitivity of csx1Δ cells to oxidative stress and their inability to induce expression of Atf1-dependent genes are partially rescued by cip1Δ and cip2Δ mutations. This study emphasizes the importance of a modulated mRNA stability in the eukaryotic stress response pathways and adds new information to the role of RNA-binding proteins in the oxidative stress response.

Published
2006
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