1. Molecular Cloning and Biochemical Characterization of Iron Superoxide Dismutase from Leishmania braziliensis.
- Author
-
Brito CCB, Maluf FV, de Lima GMA, Guido RVC, and Castilho MS
- Subjects
- Brazil, Cloning, Molecular methods, Humans, Hydrogen Peroxide pharmacology, Leishmania braziliensis drug effects, Leishmaniasis, Cutaneous drug therapy, Leishmaniasis, Cutaneous parasitology, Leishmania braziliensis genetics, Superoxide Dismutase genetics
- Abstract
Leishmaniasis is one of the most important neglected tropical diseases, with a broad spectrum of clinical manifestations. Among the clinical manifestations of the disease, cutaneous leishmaniasis, caused by species of Leishmania braziliensis, presents wide distribution in Brazil. In this work, we performed the cloning, expression, and purification of the enzyme superoxide dismutase of Leishmania braziliensis (LbSOD-B2) considered a promising target for the search of new compounds against leishmaniasis. In vitro assays based on pyrogallol oxidation showed that LbSOD-B2 is most active around pH 8 and hydrogen peroxide is a LbSOD-B2 inhibitor at low millimolar range (IC
50 = 1 mM).- Published
- 2018
- Full Text
- View/download PDF