1. Structure of a 14-3-3 coordinated hexamer of the plant plasma membrane H+ -ATPase by combining X-ray crystallography and electron cryomicroscopy.
- Author
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Ottmann C, Marco S, Jaspert N, Marcon C, Schauer N, Weyand M, Vandermeeren C, Duby G, Boutry M, Wittinghofer A, Rigaud JL, and Oecking C
- Subjects
- 14-3-3 Proteins metabolism, 14-3-3 Proteins ultrastructure, Amino Acid Motifs, Binding Sites, Cryoelectron Microscopy, Crystallography, X-Ray, Glycosides chemistry, Glycosides metabolism, Membrane Proteins metabolism, Membrane Proteins ultrastructure, Models, Biological, Models, Molecular, Mutation, Plant Proteins metabolism, Plant Proteins ultrastructure, Proton-Translocating ATPases metabolism, Proton-Translocating ATPases ultrastructure, Nicotiana metabolism, 14-3-3 Proteins chemistry, Membrane Proteins chemistry, Plant Proteins chemistry, Proton-Translocating ATPases chemistry
- Abstract
Regulatory 14-3-3 proteins activate the plant plasma membrane H(+)-ATPase by binding to its C-terminal autoinhibitory domain. This interaction requires phosphorylation of a C-terminal, mode III, recognition motif as well as an adjacent span of approximately 50 amino acids. Here we report the X-ray crystal structure of 14-3-3 in complex with the entire binding motif, revealing a previously unidentified mode of interaction. A 14-3-3 dimer simultaneously binds two H(+)-ATPase peptides, each of which forms a loop within the typical 14-3-3 binding groove and therefore exits from the center of the dimer. Several H(+)-ATPase mutants support this structure determination. Accordingly, 14-3-3 binding could result in H(+)-ATPase oligomerization. Indeed, by using single-particle electron cryomicroscopy, the 3D reconstruction of the purified H(+)-ATPase/14-3-3 complex demonstrates a hexameric arrangement. Fitting of 14-3-3 and H(+)-ATPase atomic structures into the 3D reconstruction map suggests the spatial arrangement of the holocomplex.
- Published
- 2007
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