Search

Your search keyword '"Moore, Melissa J."' showing total 7 results
Start Over Author "Moore, Melissa J." Journal molecular cell
7 results on '"Moore, Melissa J."'

7. Pre-mRNA Splicing: Awash in a Sea of Proteins

Author

Jurica, Melissa S. and Moore, Melissa J.

Subjects
*MASS spectrometry, *MESSENGER RNA, *PROTEINS
Abstract

What''s in a spliceosome? More than we ever imagined, according to recent reports employing proteomics techniques to analyze this multi-megadalton machine. As of 1999, around 100 splicing factors were identified (Burge et al., 1999); however, that number has now nearly doubled due primarily to improved purification of spliceosomes coupled with advances in mass spectrometry analyses of complex mixtures. Gratifyingly, most of the previously identified splicing factors were found in the recent mass spec studies. Nonetheless, the number of new proteins emerging with no prior connection to splicing was surprising. Without functional validation, it would be premature to label these proteins as bona fide splicing factors. Yet many were identified multiple times in complexes purified under diverse conditions or from different organisms. Another recurring theme regards the dynamic nature of spliceosomal complexes, which may be even more intricate than previously thought. [Copyright &y& Elsevier]

Published
2003
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results