1. Microsatellites fromClarias batrachusand their polymorphism in seven additional catfish species
- Author
-
Gen Hua Yue, Balázs Kovács, and László Orbán
- Subjects
Genetics ,Ecology ,biology ,Locus (genetics) ,biology.organism_classification ,Biochemistry ,Clarias ,General Biochemistry, Genetics and Molecular Biology ,Loss of heterozygosity ,Walking catfish ,Microsatellite ,Heterobranchus ,Allele ,Catfish - Abstract
We isolated 18 novel microsatellite loci from the walking catfish (Clarias batrachus), and examined their cross-amplification in seven additional catfish species from three families. Sixteen of the 18 microsatellites were polymorphic in the source species (allele number: 2–10/locus and expected heterozygosity: 0.30–0.87). Moreover, nine of these 18 primer pairs cross-amplified specific and polymorphic products from the genome of at least six of the seven other catfish species tested. However, the success rate of cross-species amplification varied from locus to locus, indicating that cross-species amplification of microsatellites is locus-dependent.
- Published
- 2003