1. Cisplatin decreases cyclin D2 expression via upregulating miR‑93 to inhibit lung adenocarcinoma cell growth
- Author
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Yan‑Mei Li, Shu‑Yang Xie, Ning Xie, Yuan‑Rong Liu, Li Pan, You‑Jie Li, Yu‑Bo Wei, Ping‑Yu Wang, and Ya‐Nan Yang
- Subjects
0301 basic medicine ,Cancer Research ,Lung Neoplasms ,cyclin D2 ,Cell ,cisplatin ,Adenocarcinoma of Lung ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,Cyclin D2 ,Genetics ,medicine ,Humans ,MTT assay ,RNA, Neoplasm ,Molecular Biology ,A549 cell ,Cisplatin ,microRNA ,Oncogene ,Chemistry ,Articles ,Cell cycle ,medicine.disease ,Neoplasm Proteins ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,lung cancer ,MicroRNAs ,cell proliferation ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,A549 Cells ,030220 oncology & carcinogenesis ,Cancer research ,Molecular Medicine ,Adenocarcinoma ,medicine.drug - Abstract
MicroRNAs (miRNAs/miRs) serve important roles in the chemotherapeutic effect of anticancer drugs. To investigate the roles of miRNAs in cisplatin‑induced suppression of lung adenocarcinoma cell proliferation, A549 cells were treated with different concentrations of cisplatin. An MTT assay demonstrated that cisplatin inhibited A549 cell proliferation in a dose‑dependent manner. Cisplatin induced cell apoptosis and inhibited cell migration by increasing the levels of miR‑93, miR‑26a and miR‑26b. Furthermore, as an upstream factor, miR‑93 was proposed to regulate cyclin D2 expression in miR‑93‑transfected A549 cells. Cisplatin also induced Bcl‑2‑associated X protein expression, and decreased that of Bcl‑2 and c‑Myc in lung adenocarcinoma cells. In vivo analysis further supported that cisplatin inhibited lung adenocarcinoma cell growth by regulating cyclin D2 and miR‑93 expression. In conclusion, our findings demonstrated that cisplatin could effectively inhibit lung adenocarcinoma cell proliferation by decreasing cyclin D2 expression via miR‑93.
- Published
- 2019