1. Mass Spectrometry–Based Analysis of Urinary Biomarkers for Dietary Tomato Intake
- Author
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Hans-Ulrich Humpf, Florian Hübner, Katharina Steinert, Yannick Hövelmann, and Lea Lewin
- Subjects
Adult ,Male ,0301 basic medicine ,Esculeogenin B ,Urine ,Mass spectrometry ,Young Adult ,03 medical and health sciences ,Alkaloids ,Urinary excretion ,Solanum lycopersicum ,Tandem Mass Spectrometry ,High doses ,Humans ,Food science ,Cross-Over Studies ,030109 nutrition & dietetics ,Chemistry ,Dietary intake ,fungi ,Reproducibility of Results ,food and beverages ,Urinary biomarkers ,Fruit and Vegetable Juices ,030104 developmental biology ,Calibration ,Dietary biomarkers ,Female ,Biomarkers ,Food Science ,Biotechnology - Abstract
Scope In this study, the applicability of several β-carboline, imidazole, and steroidal alkaloids as biomarkers for tomato juice intake is evaluated. Methods and results Over the course of a 2-week crossover dietary intervention study, 14 volunteers were given low and high doses of tomato juice after 3 days of avoiding tomato-based products. On the day of consumption and the following days, volunteers provided urine samples that were quantitatively analyzed by high-performance liquid chromatography-tandem mass spectrometry. Herein, glucose-derived β-carboline alkaloids are determined as supporting, yet non-specific dietary biomarkers for tomato juice intake. Several imidazole alkaloids represent further biomarkers, which are shown to specifically indicate consumption of tomato juice for 24 h and partly >24 h. Additionally, steroidal alkaloids derived from esculeogenin B are determined to be specific biomarkers for tomato juice detectable for at least 48 h after consumption. The intake of low and high amounts of tomato juice is significantly distinguishable based on the urinary excretion of all determined biomarkers as well. Conclusions The dietary intake of tomato juice is conclusively traceable based on urinary excretion of multiple β-carboline, imidazole, and steroidal alkaloids, and can be determined for up to 48 h after consumption. Furthermore, different intake doses can clearly be distinguished based on their urinary excretion.
- Published
- 2020