1. Proteomic comparison of needles from blister rust-resistant and susceptible Pinus strobus seedlings reveals upregulation of putative disease resistance proteins.
- Author
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Smith JA, Blanchette RA, Burnes TA, Jacobs JJ, Higgins L, Witthuhn BA, David AJ, and Gillman JH
- Subjects
- Amino Acid Sequence, Basidiomycota physiology, Electrophoresis, Gel, Two-Dimensional, Genotype, Immunity, Innate genetics, Isoelectric Point, Molecular Sequence Data, Molecular Weight, Peptides metabolism, Pinus anatomy & histology, Pinus microbiology, Plant Proteins chemistry, Proteome metabolism, Proteomics, Seedlings microbiology, Sequence Alignment, Sequence Analysis, Protein, Spectrometry, Mass, Electrospray Ionization, Up-Regulation, Disease Susceptibility metabolism, Gene Expression Regulation, Plant genetics, Pinus physiology, Plant Diseases microbiology, Plant Proteins genetics, Proteome genetics, Seedlings metabolism
- Abstract
In order to characterize a hypersensitive-like reaction in selected Pinus strobus seedlings to Cronartium ribicola, a proteomic comparison of needles from resistant and susceptible seedlings was undertaken using two-dimensional gel electrophoresis (2-DE). The results revealed 19 polypeptides specific to resistant seedlings and seven of these specific to infected resistant seedlings. There were 13 polypeptides up-regulated (> or = 3-fold increase) in resistant family P327 in comparison to needle tissue from susceptible and mock-inoculated seedlings. Electrospray ionization liquid chromatography and tandem mass spectrometry was used to sequence 11 proteins from the 2-DE gels. Sequences obtained from electrospray ionization liquid chromatography and tandem mass spectrometry were used for MS-BLAST and Pro-ID database searches allowing identification with a 95 to 99% confidence level. Six proteins were determined to be homologs of proteins with known roles in disease resistance, five were determined to be homologs of members of the leucine-rich repeat (LRR) superfamily, and one was a homolog of heat shock protein 90, a protein that serves as a cofactor for certain LRR proteins. This is the first report of members of the LRR family with functional homologs in Pinus strobus and of a molecular basis for white pine blister rust resistance in Pinus strobus.
- Published
- 2006
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